James left Sunday's tilt against the Toronto Raptors after experiencing soreness. The four-time NBA Finals MVP sprained the same ankle in March and was sidelined for 20 games.
James registered 17.5 points, 7.5 boards, and 6.5 assists per contest during his two-game return, but he also averaged five turnovers.
Los Angeles enters Thursday just a half-game ahead of the Portland Trail Blazers for the No. 6 seed in the West.