Woods - whose design firm was the architect behind the newly opened Payne's Valley course at Big Cedar Lodge in Ridgedale, Missouri - and Thomas got off to a slow start. They lost the opening point in the best-ball format, which was played for six holes.
Woods and Thomas then took the alternate-shot point before the teams split singles match points. Thomas beat McIlroy - who lipped out for birdie on three straight holes - and Rose defeated Woods, resulting in a 2-2 tie.
However, the tiebreaker took place at the unique 19th hole at Payne's Valley. Thomas hit the ball closest to the pin on the 123-yard par 3 with an island green, which ultimately sealed the victory.
Here's how the charity match ended, with appearances from legends Jack Nicklaus and Gary Player:
All money raised during the competition will be donated to the Payne Stewart Family Foundation, which helps support organizations including Kids Across America, the American Junior Golf Association, and The First Tee.