Golladay, who was also sidelined in the season opener, didn't practice this week.
The 26-year-old made his first Pro Bowl after leading the NFL with 11 receiving touchdowns last season. He also recorded 65 receptions and 1,190 yards.
On Friday, Detroit also ruled out veteran cornerback Desmond Trufant for Week 2 due to a hamstring injury.
The Lions, however, removed cornerback Jeff Okudah from the injury report. The 2020 third overall pick is expected to be on the field for his NFL debut Sunday after missing last week's game with a hamstring issue.