Following a sensational curling effort from James Rodriguez that opened the scoring after 36 minutes, both teams exchanged three goals within a hectic six-minute span in the second half that saw the lead change hands twice.
A quickfire double courtesy of Kellyn Acosta and Bobby Wood gave the U.S. a dramatic lead just short of the hour mark, but Carlos Bacca restored parity just three minutes later by converting Santiago Arias' low cross.
Monaco striker Radamel Falcao later buried Colombia's third goal of the contest in the 74th minute, capitalizing on a rapid counterattack that left much of the American defense trailing in the opposite half. Miguel Borja's overhead kick all but sealed the win for the visitors with 11 minutes remaining.
The U.S. will now prepare to face another South American World Cup contestant when they host Peru in East Hartford, Conn. on Tuesday. Colombia, meanwhile, will prepare to face Costa Rica on the same day at the Red Bull Arena in Harrison, N.J.