Jones is dealing with a calf injury and was unable to practice all week. He'll be sidelined for the second straight game.
The 25-year-old popped up on the injury report following Green Bay's Week 6 loss to the Tampa Bay Buccaneers.
Jamaal Williams and rookie A.J. Dillon will lead the Packers' backfield against their NFC North rivals. Williams carried the ball 19 times for 77 yards last week against the Houston Texans, adding four catches for 34 yards.
Jones has rushed for 389 yards and five touchdowns over his first five games, averaging 5.2 yards per carry.